《植物生理学报》 2016, 52(1): 117-124

二甲基亚砜对拟南芥基因组DNA甲基化的影响

李忠爱, 杜亚琼, 李杰, 王子成^*

河南大学农业技术研究所, 河南开封475004

通信作者：王子成；E-mail: wzc@henu.edu.cn

摘　要：

本文以拟南芥(Arabidopsis thaliana)为材料, 研究不同浓度二甲基亚砜(DMSO)处理下拟南芥幼苗生长发育及基因组DNA的甲基化水平和变化模式。结果表明, 130、260、400和500 μmol•L^-1 DMSO处理影响拟南芥幼苗的根长、株高以及抽薹和开花时间; 甲基化敏感扩增多态性(methylation sensitive amplification polymorphism, MSAP)分析表明, 经130、260、400和500 μmol•L^-1 DMSO处理后基因组DNA甲基化比率分别为24.26%、14.36%、16.58%和13.02%, 对照为 22.50%。即拟南芥经DMSO处理后存在基于DNA甲基化水平和模式改变的表观遗传变异, 5-甲基胞嘧啶百分含量的变化与DMSO处理浓度之间存在显著的剂量效应关系。与对照相比, 130、260、400和500 μmol•L^-1 DMSO处理下拟南芥幼苗基因组DNA的甲基化和去甲基化分别为7.21%、6.03%、11.62%、10.20%和6.25%、10.05%、8.08%、8.67%。

关键词：拟南芥; 二甲基亚砜; DNA甲基化; MSAP

收稿：2015-10-08   修定：2016-01-01

资助：国家自然科学基金(31171982、31372090)和河南大学科研基金(xxjc. 20140008)。

Effects of dimethyl sulfoxide on Arabidopsis thaliana DNA methylation

LI Zhong-Ai, DU Ya-Qiong, LI Jie ,WANG Zi-Cheng^*

Institute of Agricultural Biotechnology, Henan University, Kaifeng, Henan 475004, China

Corresponding author: WANG Zi-Cheng; E-mail: wzc@henu.edu.cn

Abstract:

The objectives of this research were to assess the effect of dimethyl sulfoxide (DMSO) stress on the development as well as genomic DNA methylation levels and patterns of Arabidopsis thaliana seedlings. Dimethyl sulfoxide at concentrations of 130, 260, 400 and 500 μmol•L^-1 affected Arabidopsis thaliana seedlings root length, plant height, bolting and flowering time. Methylation sensitive amplification polymorphism (MSAP) analysis showed that genomic DNA methylation levels of dimethyl sulfoxide stressed seedlings were lower than that of the untreated plants. The results indicated that dimethyl sulfoxide stress induced epigenetic variations in A. thaliana seedlings, and DMSO treatment concentration had significant dose effect relationship with the change of 5-methylcytosine percentage. At dimethyl sulfoxide concentrations of 130, 260, 400 and 500 μmol•L^-1, methylation and demethylation of A. thaliana seedling genomic DNA were respectively 7.21%, 6.03%, 11.62%, 10.20% and 6.25%, 10.05%, 8.08% , 8.67%.

Key words: Arabidopsis thaliana; dimethyl sulfoxide; DNA methylation; MSAP